Biomolecular Films (Surfactant Science Series) by James F. Rusling

By James F. Rusling

Surveying special and sleek biomolecular movie methodologies and investigative strategies, this article examines motion pictures of biomolecules which can supply reliable surfaces for catalyzing enzyme reactions, serve in biosensors and as biorecognition components, mediate nanoparticle formation, and supply a foundation for primary reports and purposes in biomedicine and biomedical units. The authors talk about designing practical biomolecular motion pictures on electrodes, biomimetic membranes on steel helps, peptide and protein-based biomolecular assemblies, floor plasmon resonance spectroscopy, and biosensors.

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The NiFeS enzyme hydrogenase from Desulfovibrio dusulfuricans Norway was adsorbed directly onto basal-plane pyrolytic graphite, allowing studies of its reaction with cyt c3 with a lifetime of the hydrogenase ¢lm of several hours [92]. Megasphaera elsdenii Fe hydrogenase was adsorbed from solution at submonolayer coverage onto rough glassy carbon electrodes [93]. Although no direct electron transfer peaks were found, electron transfer involving this enzyme was con¢rmed by catalytic currents for reduction of Hþ and oxidation of H2.

100À200 mV, were needed to extract reliably selfconsistent values of reorganization energies. An example of a ¢t is shown in Fig. 7. The mean values of reorganization energies for reduced and oxidized forms of Mb, respectively, found by this method were lRED ¼ 0:41 eV and lOX ¼ 0:21 eV. FIG. 7 Experimental current (s)with model background current subtracted resulting from regression analysis of SWV data at 200-Hz frequency, 160-mV pulse height shown with the best-fit line from the Marcus model.

Nonlinear regression analysis of SWV allowed direct estimation of electron-transfer rate constants and reorganization energies. , 100À200 mV, were needed to extract reliably selfconsistent values of reorganization energies. An example of a ¢t is shown in Fig. 7. The mean values of reorganization energies for reduced and oxidized forms of Mb, respectively, found by this method were lRED ¼ 0:41 eV and lOX ¼ 0:21 eV. FIG. 7 Experimental current (s)with model background current subtracted resulting from regression analysis of SWV data at 200-Hz frequency, 160-mV pulse height shown with the best-fit line from the Marcus model.

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