Biofertilizer Manual by Dr. Pham Van Toan

By Dr. Pham Van Toan

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Soil Sci. 2. P-solubilizing Activity 32 P and 33 P are two radioisotopes suitable for agronomic studies. A). A. of sample requires 2 independent measurements: 1. Determination of activity of radioisotope by radioassay technique using appropriate detector (proportional detector, Geiger-Mueller detector, liquid scintillation counting, Cerenkov counting or sodium-iodide scintillation detector). 2. g. spectrophotometric method. 32P in general is used to study the utilization of P fertilizer by crops in greenhouse and field experiments.

20 right. 26 Usually peak height of 15 N14N and 14 N2 (14N14N) are measured as shown in Fig. 18. The background of 14N2 is baseline of the chart, however, the background of 15N14N is the front of the peak as shown by “d” in Fig. 18. 7 nm) increase and disturb the back ground level of 15N14N. The height of background of 15N14N (d-b) should be lower than the peak height (c-d). Calculation of N derived from labeled N and fertilizer efficiency The percentage of N derived from labeled N can be calculated by the equation as follows: 100 x atom % excess of the sample / atom % excess of the labeled source.

The clear solution is then collected by the filteration or centrifugation. The water soluble phosphorus in the clear solution is than determinated by spectrophotometric method. Reference 1. , 1990 Ureide assay for measuring nitrogen fixation by nodulated soybean calibrated by 15N methods, Plant Physiol. 93, 495-503 2. , 1964 Physiological studies on the nutrition of soybean of soybean plants. 2. Effect of nodule formation on nitrogenous constituents of soybean J. Soil Sci. Manure, Japan, 35, 323-327 3.

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